The Laboratory for Centrosome and Cytoskeleton Biology  

Institute of Human Genetics, Heinrich-Heine-Universität Düsseldorf 


  • Gabriel, E., Albanna, W., Pasquini, G., Ramani, A., Josipovic, N., Mariappan, A., Schinzel, F., Karch, C.M., Bao, G., Gottardo, M., et al. Gopalakrishnan J* (2021). Human brain organoids assemble functionally integrated bilateral optic vesicles. Cell Stem Cell. 2021 Aug 12:S1934-5909(21)00295-2. doi: 10.1016/j.stem.2021.07.010.


  • Goranci-Buzhala, G., Mariappan, A., Ricci-Vitiani, L., Josipovic, N., Pacion, S., Gottardo, M., Ptok, J., Callaini, G., Rajalingam, K., Dynlacht, B., et al. Gopalakrishnan J*.Cilium induction triggers the differentiation of glioma stem cells. Cell Reports. Sep 7;36(10):109656. doi: 10.1016/j.celrep.2021.109656.


  • Ramani A, Pranty AI, Gopalakrishnan J*. Neurotropic effects of SARS-CoV-2 modeled by the Human Brain Organoids. Stem Cell Reports. 2021 Mar 9;16(3):373-384.



  • Mariappan A, Goranci-Buzhala G, Ricci-Vitiani L, Pallini R , Gopalakrishnan J*. Trends and challenges in modeling glioma using 3D human brain organoids. Cell Death Differ. 2020 Dec 1:1-9. doi: 10.1038/s41418-020-00679-7.


  • Farooq M [...] Gopalakrishnan J [..] Christensen ST, Larsen LA. RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis. Nat Commun. 2020 Nov 16;11(1):5816. doi: 10.1038/s41467-020-19658-0.


  • Anand Ramani, Lisa Muller, Philipp Niklas Ostermann [...] Heiner Schaal* and Gopalakrishnan J*. SARS-CoV-2 targets neurons of 3D human brain organoids. EMBO Journal. 2020 Oct 15;39(20):e106230. doi: 10.15252/embj.2020106230. (Cover article)


  • Goranci-Buzhala G, Mariappan A, Gabriel E, Ramani A, Ricci-Vitiani L, Buccarelli M, D'Alessandris QG, Pallini R, Gopalakrishnan J*. Rapid and efficient invasion assay of glioblastoma in human brain organoids. Cell Rep. 2020. Jun 9;31(10):107738. doi: 10.1016/j.celrep.2020.107738


  • Gabriel E, Ramani A, Altinisik N, Gopalakrishnan J*. Human brain organoids to decode mechanisms of microcephaly. Front Cell Neurosci. 2020 May 8;14:115. doi: 10.3389/fncel.2020.00115. eCollection 2020.


  • Liu W et al, Gopalakrishnan J and Pandyra AA*. Repurposing the serotonin agonist Tegaserod as an anticancer agent in melanoma: Molecular mechanisms and clinical implications. J Exp Clin Cancer Res. 2020 Feb 21;39(1):38.



  • Lapart JA et al, Gopalakrishnan J and Durand B*. Dzip1 and Fam92 form a ciliary transition zome complex with cell-type-specific roles in Drosophila.  Elife. 2019 Dec 10;8 pii: e49307


  • Gopalakrishnan J*. The Emergence of Stem Cell-Based Brain Organoids: Trends and Challenges.  Bioessays. 2019 Aug;41(8):e1900011. doi: 10.1002/bies.201900011.


  • Arul Mariappan Soni K, Schorpp K, Zhao F, Minakar A, Zheng X, Mandad S, Macheleidt I, Ramani A, Kubelka T, Dawidowski M, Golfmann K, Wason A, Yang C, Simons J, Schmalz HG, Hyman AA, Aneja R, Ullrich R, Urlaub H, Odenthal M, Büttner R, Li H, Sattler M, Hadian K, Gopalakrishnan J*. Inhibition of CPAP-Tubulin interaction prevents the proliferation of centrosome amplified cancer cells. EMBO Journal  2019. Dec 10. pii: e99876. doi: 10.15252/embj.201899876

    • Highlighted in EMBO J as Magic Bullet



  • Ramani A, Mariappan A, Gottardo M, Mandad S, Urlaub H, Avidor-Reiss T, Riparbelli M, Callaini G, Debec A, Feederle R,et al., Gopalakrishnan J*. Plk1/Polo phosphorylates Sas-4 at the onset of mitosis for an efficient recruitment of pericentriolar material to centrosomes. Cell Reports,  2018. Dec 26;25(13):3618-3630.e6 (2018).


  • Lilian A Martinez Carrera, Elke Gabriel, Colin Donohoe, Irmgard Hölker, Aruljothi Mariappan, Markus Storbeck, Mirka Uhlirova, Jay Gopalakrishnan, Brunhilde Wirth* Novel insights into SMALED2: BICD2 mutations increase microtubule stability and cause defects in axonal and NMJ development.  Human Molecular Genetics, 2018. May 15;27(10):1772-1784



  • Goranci-Buzhala G, Gabriel E, Mariappan A, Gopalakrishnan J* Losers of Primary Cilia Gain the Benefit of survival. Cancer Discovery 12, 2017. 1374-1375.


  • Gabriel E, Gopalakrishnan J* Generation of iPSC-derived human brain organoids to model early neurodevelopmental disorders. J Vis Exp. 14; 2017. (122).


  • Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, Korhonen E, Smura T, Vapalahti O, Papantonis A, Schmidt-Chanasit J, Riparbelli M, Callaini G, Krönke M, Utermöhlen O, Gopalakrishnan J*. Recent Zika virus isolates induce premature differentiation of neural progenitors in human brain organoids. Cell Stem Cell 20, 2017. 1-10.



  • Li Ming Gooi and Gopalakrishnan J* Regulation of stem cells in their nicheCurrent Stem Cell Reports, 3, 3, 2016. 282-289.


  • Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J*, Li H*. Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length. Nat Commun 7, 2016. 11874.


  • Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, Šarić T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J*. CPAP promotes timely cilium disassembly to maintain neural progenitor pool.  EMBO Journal. 2016. Apr 15;35(8):803-19. (Cover article)


  • Wike CL et al., Jay Gopalakrishnan and Tyler JK*. Excess-free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans. Cell Cycle. 2016 Jun 1:0.


  • Brenke et al., and Jay Gopalakrishnan, Hadian K* Identification of Small Molecule Frequent Hitters of GST–Glutathione interaction. J Biomol Screen. 6 Apr 4. 2016.



  • Persefoni Thomopoulou et al., Gopalakrishnan J and Hans-Günther Schmalz*. New Colchicine-Derived Triazoles and Their Influence on Cytotoxicity and Microtubule Morphology. ACS Med. Chem. LettACS Med Chem Lett. 2015. Dec 29;7(2):188-91.


  • Anand Ramani, Li Ming Gooi and Gopalakrishnan J*. Building the meat of pericentriolar material surrounding the skeleton of a centriole. Cell News 2/2015, German Society for Cell Biology (DGZ)



  • Zheng X, Gooi LM, Wason A, Gabriel E, Mehrjardi NZ, Yang Q, Zhang X, Debec A, Basiri ML, Avidor-Reiss T, Pozniakovsky A, Poser I, Saric T, Hyman AA, Li H*, Gopalakrishnan J* Conserved TCP  domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis. PNAS, 2014. Volume 111, Number 3.


  • Pannu V, Rida PC, Celik B, Turaga RC, Ogden A, Cantuaria G, Jay Gopalakrishnan, Aneja R*. Centrosome-declustering drugs mediate a two-pronged attack on interphase and mitosis in supercentrosomal cancer cells.  Cell Death Dis. 2014. Nov 20;5:e1538. doi: 10.1038/cddis.2014.505.



  • Avidor-Reiss T, and Jay Gopalakrishnan. Cell Cycle Regulation of the Centrosome and Cilium.  Drug Discov Today Dis Mech. 2013. Dec 1;10(3-4):e119-e124


  • Avidor-Reiss T, and Gopalakrishnan J Building a centrioleCurr Opin Cell Biol; 2013. S0955-0674(12)00180-9.


  • Schorpp K, Rothenaigner I, Salmina E, Reinshagen J, Low T, Brenke JK, Gopalakrishnan J, Tetko IV, Gul S, Hadian K*. Identification of Small-Molecule Frequent Hitters from AlphaScreen High-Throughput Screens. J Biomol Screen. Dec 26, (2013).



  • Gopalakrishnan J, Chim YC, Ha A, Basiri ML, Lerit DA, Rusan NM, and Avidor-Reiss T. Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat Cell Biol 14, 865-873.



  • Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, and Avidor-Reiss T . Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun. 2011. 2, 359.



  • Gopalakrishnan J, Guichard P, Smith AH, Schwarz H, Agard DA, Marco S, and Avidor-Reiss T. Self-assembling SAS-6 Multimer Is a Core Centriole Building Block. Journal of Biological Chemistry. 2010. 285, 8759-8770.



  • Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, Malicki J, and Avidor-Reiss T (2008). Drosophila asterless and Vertebrate Cep152 Are Orthologs Essential for Centriole Duplication. Genetics. 2008. 180, 2081-2094.



  • Gopalakrishnan J, Görisch H, Adrian L. Studies on hydrogenase activity and chlorobenzene respiratio in Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2004) Dec;182(6):498-504.


  • Gopalakrishnan J, Görisch H, Adrian L. Dehalorespiration with hexachlorobenzene and pentachlorobenzene by Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2003) Dec;180(6):411-6.



Book chapters:

  • T Avidor-Reiss, J Gopalakrishnan, ML Basiri. Sas-4-mediated PCM assembly is regulated by Tubulin Dimers. MOLECULAR BIOLOGY OF THE CELL 23. (2012)


  • G Ben-David, J Gopalakrishnan, T Avidor-Reiss. SAS-6 Tetramers Self-assemble in the Formation of the Centriole Central Tubule in Drosophila IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47, S43-S44 (2011)


  • J Gopalakrishnan, YC Chim, A Ha, M Basiri, T Avidor-Reiss. A Sas-4/tubulin module regulates the formation of centrosomal complexes and centrosome biogenesis. MOLECULAR BIOLOGY OF THE CELL 22 (2011).


  • T Avidor-Reiss, J Gopalakrishnan, S Blachon, A Polyanovsky. Centriole duplication and inheritance in Drosophila melanogaster. The Centrosome, 3-31 6 (2012).