The laboratory for centrosome and cytoskeleton biology

Center for molecular medicine, University of Cologne, Germany



  • Lilian A Martinez Carrera, Elke Gabriel, Colin Donohoe, Irmgard Hölker, Aruljothi Mariappan, Markus Storbeck, Mirka Uhlirova, Jay Gopalakrishnan, Brunhilde Wirth* Novel insights into SMALED2: BICD2 mutations increase microtubule stability and cause defects in axonal and NMJ development. Human Molecular Genetics, Published online: 08 March 2018 (2018).


  • Goranci-Buzhala G, Gabriel E, Mariappan A, Gopalakrishnan J* Losers of Primary Cilia Gain the Benefit of survival. Cancer Discovery 12, 1374-1375 (2017).
  • Gabriel E, Gopalakrishnan J* Generation of iPSC-derived human brain organoids to model early neurodevelopmental disorders. J Vis Exp. 14;(122) (2017).
  • Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, Korhonen E, Smura T, Vapalahti O, Papantonis A, Schmidt-Chanasit J, Riparbelli M, Callaini G, Krönke M, Utermöhlen O, Gopalakrishnan J*. Recent Zika virus isolates induce premature differentiation of neural progenitors in human brain organoids. Cell Stem Cell 20, 1-10  (2017).


  • Li Ming Gooi and Gopalakrishnan J* Regulation of stem cells in their niche. Current Stem Cell Reports, 3, 3, 282-289 (2016).
  • Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J*, Li H*. Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length. Nat Commun 7, 11874 (2016).
  • Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, Šarić T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J*. CPAP promotes timely cilium disassembly to maintain neural progenitor pool. EMBO Journal. Apr 15;35(8):803-19. (Cover article) (2016).
  • Wike CL et al., Jay Gopalakrishnan and Tyler JK*. Excess free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans. Cell Cycle. 2016 Jun 1:0 (2016).
  • Brenke et al., and Jay Gopalakrishnan, Hadian K* Identification of Small Molecule Frequent Hitters of GST–Glutathione interaction. J Biomol Screen. 6 Apr 4 (2016).


  • Persefoni Thomopoulou et al., Jay Gopalakrishnan and Hans-Günther Schmalz* (2015). New Colchicine-Derived Triazoles and Their Influence on Cytotoxicity and Microtubule Morphology. ACS Med. Chem. Lett. ACS Med Chem Lett. Dec 29;7(2):188-91, (2015).
  • Anand Ramani, Li Ming Gooi and Gopalakrishnan J*. Building the meat of pericentriolar material surrounding the skeleton of a centriole. Cell News 2/2015, German Society for Cell Biology (DGZ) (2015).


  • Zheng X, Gooi LM, Wason A, Gabriel E, Mehrjardi NZ, Yang Q, Zhang X, Debec A, Basiri ML, Avidor-Reiss T, Pozniakovsky A, Poser I, Saric T, Hyman AA, Li H*, Gopalakrishnan J* Conserved TCP  domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis. PNAS, Volume 111, Number 3. (2014).
  • Pannu V, Rida PC, Celik B, Turaga RC, Ogden A, Cantuaria G, Jay Gopalakrishnan, Aneja R*. Centrosome-declustering drugs mediate a two-pronged attack on interphase and mitosis in supercentrosomal cancer cells. Cell Death Dis. Nov 20;5:e1538. doi: 10.1038/cddis.2014.505 (2014).


  • Avidor-Reiss T, and Jay Gopalakrishnan. Cell Cycle Regulation of the Centrosome and Cilium. Drug Discov Today Dis Mech. Dec 1;10(3-4):e119-e124 (2013).
  • Avidor-Reiss T, and Gopalakrishnan J Building a centriole. Curr Opin Cell Biol; S0955-0674(12)00180-9. (2013).
  • Schorpp K, Rothenaigner I, Salmina E, Reinshagen J, Low T, Brenke JK, Jay Gopalakrishnan, Tetko IV, Gul S, Hadian K*. Identification of Small-Molecule Frequent Hitters from AlphaScreen High-Throughput Screens. J Biomol Screen. Dec 26, (2013).


  • Gopalakrishnan J, Chim YC, Ha A, Basiri ML, Lerit DA, Rusan NM, and Avidor-Reiss T (2012). Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat Cell Biol 14, 865-873.


  • Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, and Avidor-Reiss T (2011). Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun 2, 359.


  • Gopalakrishnan J, Guichard P, Smith AH, Schwarz H, Agard DA, Marco S, and Avidor-Reiss T (2010). Self-assembling SAS-6 Multimer Is a Core Centriole Building Block. Journal of Biological Chemistry 285, 8759-8770.


  • Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, Malicki J, and Avidor-Reiss T (2008). Drosophila asterless and Vertebrate Cep152 Are Orthologs Essential for Centriole Duplication. Genetics 180, 2081-2094.


  • 21. Gopalakrishnan J, Görisch H, Adrian L. Studies on hydrogenase activity and chlorobenzene respiratio in Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2004) Dec;182(6):498-           504.
  • 22. Gopalakrishnan J, Görisch H, Adrian L. Dehalorespiration with hexachlorobenzene and pentachlorobenzene by Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2003) Dec;180(6):411-6.
  • Book chapters:
  • 1. T Avidor-Reiss, J Gopalakrishnan, ML Basiri. Sas-4-mediated PCM assembly is regulated by Tubulin Dimers. MOLECULAR BIOLOGY OF THE CELL 23. (2012).
  • 2. G Ben-David, J Gopalakrishnan, T Avidor-Reiss. SAS-6 Tetramers Self-assemble in the Formation of the Centriole Central Tubule in Drosophila IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47, S43-S44 (2011).
  • 3. J Gopalakrishnan, YC Chim, A Ha, M Basiri, T Avidor-Reiss. A Sas-4/tubulin module regulates the formation of centrosomal complexes and centrosome biogenesis. MOLECULAR BIOLOGY OF THE CELL 22 (2011).
  • 4. T Avidor-Reiss, J Gopalakrishnan, S Blachon, A Polyanovsky. Centriole duplication and inheritance in Drosophila melanogaster. The Centrosome, 3-31 6 (2012).